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Product Info Summary
| SKU: | A06368 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Spred2 Antibody
SKU/Catalog Number
A06368
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Spred2 Antibody (Catalog # A06368). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Spred2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Spred2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06368)
Host
Rabbit
Contents
Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2. The immunogen is located within amino acids 140 - 190 of Spred2.
Cross-reactivity
This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.
Reactive Species
A06368 is reactive to SPRED2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
47.6 kDa
Background of SPRED2
Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06368 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-Spred2 Antibody (A06368)
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