Anti-Sprouty 4 Rabbit Monoclonal Antibody

Product Name

Anti-Sprouty 4 Rabbit Monoclonal Antibody

View all Spry4 Antibodies

SKU/Catalog Number

M04343

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Sprouty 4 Rabbit Monoclonal Antibody catalog # M04343. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Sprouty 4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04343)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

18S63

Isotype

IgG

Immunogen

A synthesized peptide derived from human Sprouty 4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04343 is reactive to Spry4 in Human, Mouse, Rat

Applications

M04343 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

32.523kDa

Background of Spry4

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of Sprouty 4 expression in MCF7 cell lysate.

Gene/Protein Information For Spry4 (Source: Uniprot.org, NCBI)

Alternative Names

protein sprouty homolog 4; sprouty homolog 4 (Drosophila); SPRY4; spry-4 Spry4|A030006O18Rik, spro, sprouty4|sprouty RTK signaling antagonist 4|protein sprouty homolog 4

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