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Product Info Summary
| SKU: | M03376 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SPTA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03376
BM5762 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SPTA1 Rabbit Monoclonal Antibody catalog # M03376. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03376)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFGF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SPTA1
Reactive Species
M03376 is reactive to SPTA1 in Human
Observed Molecular Weight
280 kDa
Calculated molecular weight
280.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03376 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of SPTA1 expression in K562 cell lysate.
Specific Publications For Anti-SPTA1 Rabbit Monoclonal Antibody (M03376)
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6 Customer Q&As for Anti-SPTA1 Rabbit Monoclonal Antibody
Question
We are currently using anti-SPTA1 Monoclonal antibody M03376 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-SPTA1 Monoclonal antibody (M03376) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
What sample was used in your WB analysis of M03376?
Verified customer
Asked: 2020-01-07
Answer
For the Anti-SPTA1 Rabbit Monoclonal Antibody (M03376), Western blot analysis of SPTA1 expression in K562 cell lysate.
Boster Scientific Support
Answered: 2020-01-14
Question
Does anti-SPTA1 Monoclonal antibody M03376 work on zebrafish WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Our lab technicians have not validated anti-SPTA1 Monoclonal antibody M03376 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-SPTA1 Monoclonal antibody M03376 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this M03376 anti-SPTA1 Monoclonal antibody reactive to the isotypes of SPTA1?
B. Collins
Verified customer
Asked: 2018-01-30
Answer
The immunogen of M03376 anti-SPTA1 Monoclonal antibody is A synthesized peptide derived from human SPTA1 Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-30
Question
Would M03376 anti-SPTA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
You can see on the product datasheet, M03376 anti-SPTA1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-06
Question
Is a blocking peptide available for product anti-SPTA1 Monoclonal antibody (M03376)?
A. Lewis
Verified customer
Asked: 2017-03-17
Answer
We do provide the blocking peptide for product anti-SPTA1 Monoclonal antibody (M03376). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-03-17
