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Product Info Summary
| SKU: | M00107-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Src Rabbit Monoclonal Antibody
SKU/Catalog Number
M00107-1
BM4298 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Src Rabbit Monoclonal Antibody catalog # M00107-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Src Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00107-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DCI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human
Reactive Species
M00107-1 is reactive to SRC in Human, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
59.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00107-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of Src expression in U87-MG cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-Src Rabbit Monoclonal Antibody (M00107-1)
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4 Customer Q&As for Anti-Src Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-Src Rabbit Monoclonal antibody. However we have seen positive staining in leukemic t-cell cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
From what I have seen in literature, leukemic t-cell does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-05-04
Question
We ordered your anti-Src Rabbit Monoclonal antibody for IF on cervix carcinoma last year. I am using rat, and We are going to use the antibody for WB next. We need examining cervix carcinoma as well as fundus of stomach in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
I looked at the website and datasheets of our anti-Src Rabbit Monoclonal antibody and it appears that M00107-1 has been validated on rat in both IF and WB. Thus M00107-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-30
Question
We have seen staining in human fundus of stomach. Any tips? Is anti-Src Rabbit Monoclonal antibody supposed to stain fundus of stomach positively?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
According to literature fundus of stomach does express SRC. According to Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-Src Rabbit Monoclonal antibody M00107-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?
A. Singh
Verified customer
Asked: 2013-11-27
Answer
The anti-Src Rabbit Monoclonal antibody (M00107-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-27
