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Product Info Summary
| SKU: | A01678 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SREBF2 Antibody
SKU/Catalog Number
A01678
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SREBF2 Antibody (Catalog # A01678). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SREBF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SREBF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01678)
Host
Rabbit
Contents
SREBF2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2. The immunogen is located within amino acids 440 - 490 of SREBF2.
Cross-reactivity
SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1.
Reactive Species
A01678 is reactive to SREBF2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
123.7 kDa
Background of SREBF2
The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1,2). The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2). SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01678 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.
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Immunohistochemistry of SREBF2 in human spleen tissue with SREBF2 antibody at 5 μg/mL.
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Immunofluorescence of SREBF2 in human spleen tissue with SREBF2 antibody at 20 μg/mL.
Specific Publications For Anti-SREBF2 Antibody (A01678)
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