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Product Info Summary
| SKU: | A10454 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SRPX1 Antibody
SKU/Catalog Number
A10454
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SRPX1 Antibody (Catalog # A10454). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SRPX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10454)
Host
Rabbit
Contents
SRPX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SRPX1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human SRPX1. The immunogen is located within amino acids 30 - 80 of SRPX1.
Reactive Species
A10454 is reactive to SRPX in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
51.6 kDa
Background of SRPX
SRPX1 was initially identified as a candidate gene for X-linked retinitis pigmentosa and is overexpressed in the trabecular network of the eye in glaucoma conditions. Its expression is significantly downregulated in a number cancer cell lines and malignant tumor tissues and its ectopic expression induces apoptosis through the endoplasmic reticulum and the activation of caspase-12, -9, and -3, indicating that it can function as a tumor suppressor. SRPX1 is also involved with the regulation of autophagy under low serum conditions and can associate with Rab24, a member of the Rab GTPase protein family that is involved in autophagy. This association of SRPX1 with Rab24 is enhanced during autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10454 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SRPX1 antibody can be used for detection of SRPX1 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SRPX1 in SK-N-SH cell lysate with SRPX1 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of SRPX1 in human brain tissue with SRPX1 antibody at 5 μg/mL.
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Immunofluorescence of SRPX1 in human brain tissue with SRPX1 antibody at 20 μg/mL.
Specific Publications For Anti-SRPX1 Antibody (A10454)
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