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Product Info Summary
| SKU: | A03127 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SRSF3 Antibody
SKU/Catalog Number
A03127
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SRSF3 Antibody (Catalog # A03127). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SRSF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SRSF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03127)
Host
Rabbit
Contents
SRSF3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SRSF3 antibody was raised against a 19 amino acid peptide near the center of human SRSF3. The immunogen is located within amino acids 80 - 130 of SRSF3.
Cross-reactivity
SRSF3 antibody is human, mouse and rat reactive.
Reactive Species
A03127 is reactive to SRSF3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
19.3 kDa
Background of SRSF3
SRSF3, a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome, may be involved in RNA processing in relation with cellular proliferation and/or maturation (1,2). Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins (3). The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03127 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL.
For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL .
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Immunofluorescence of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL.
Red: SRSF3 Antibody (A03127)
Blue: DAPI staining
Specific Publications For Anti-SRSF3 Antibody (A03127)
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