Anti-ST3gal6 Antibody

Product Name

Anti-ST3gal6 Antibody

View all ST3GAL6 Antibodies

SKU/Catalog Number

A09513

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ST3gal6 Antibody (Catalog # A09513). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ST3gal6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ST3gal6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09513)

Host

Rabbit

Contents

ST3gal6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

ST3gal6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ST3gal6. The immunogen is located within amino acids 230 - 280 of ST3gal6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.

Reactive Species

A09513 is reactive to ST3GAL6 in Human, Mouse, Rat

Applications

A09513 is guaranteed for ELISA, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.214kDa

Background of ST3GAL6

Sialyltransferases catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures, such as the sialyl-Lewis X determinants, and are widely distributed in many cell types. However, cancer cells often express more heavily sialylated glycans on their cell surface and this feature sometimes correlates with invasiveness. In contrast, expression of ST3gal6, a member of the sialyltransferase family that sialylates type II lactosamine structures on glycoproteins and glycolipids, was found to be significantly decreased by hypermethylation of the gene in gastrointestinal cancer. At least three isoforms of ST3gal6 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ST3gal6 antibody can be used for detection of ST3gal6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.

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Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.

Gene/Protein Information For ST3GAL6 (Source: Uniprot.org, NCBI)

Alternative Names

alpha2,3-sialyltransferase; CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI; EC 2.4.99; EC 2.4.99.-; EC 2.4.99.9; Sialyltransferase 10; SIAT10; ST3 beta-galactoside alpha-2,3-sialyltransferase 6; ST3Gal VI; ST3GAL6; ST3GALVI; ST3GALVIsialyltransferase 10 (alpha-2,3-sialyltransferase VI); type 2 lactosamine alpha-2,3-sialyltransferase ST3GAL6 SIAT10, ST3GALVI ST3 beta-galactoside alpha-2,3-sialyltransferase 6 type 2 lactosamine alpha-2,3-sialyltransferase|CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI|alpha2,3-sialyltransferase ST3Gal VI|sialyltransferase 10 (alpha-2,3-sialyltransferase VI)

For more info on ST3GAL6, check out the ST3GAL6 Infographic

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In this infographic, you will see the following information for ST3GAL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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