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Product Info Summary
| SKU: | A10348 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ST6GALNAC2 Antibody Picoband™
View all ST6 Sialyltransferase 2/ST6GALNAC2 Antibodies
SKU/Catalog Number
A10348
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ST6GALNAC2 Antibody Picoband™ catalog # A10348. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ST6GALNAC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10348)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ST6GALNAC2 recombinant protein (Position: Q32-R374).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10348 is reactive to ST6GALNAC2 in Human, Mouse, Rat
Applications
A10348 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
41.939kDa
Background of ST6 Sialyltransferase 2/ST6GALNAC2
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 is an enzyme that in humans is encoded by the ST6GALNAC2 gene. ST6GALNAC2 belongs to a family of sialyltransferases that add sialic acids to the nonreducing ends of glycoconjugates. At the cell surface, these modifications have roles in cell-cell and cell-substrate interactions, bacterial adhesion, and protein targeting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ST6GALNAC2 using anti-ST6GALNAC2 antibody (A10348).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST6GALNAC2 antigen affinity purified polyclonal antibody (Catalog # A10348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST6GALNAC2 at approximately 40 kDa. The expected band size for ST6GALNAC2 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ST6GALNAC2 (Source: Uniprot.org, NCBI)
Gene Name
ST6GALNAC2
Full Name
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Weight
41.939kDa
Superfamily
glycosyltransferase 29 family
Alternative Names
(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2; EC 2.4.99.-; FLJ45660; GalNAc alpha-2,6-sialyltransferase II; SAITL1; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase); Sialyltransferase 7B; sialyltransferase ST6GALNAC2 SAITL1, SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2|(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B|(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase|SIAT7-B|ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2|ST6 GalNAc alpha-2,6-sialyltransferase 2|ST6GALNAC2, alpha-2,6-sialyltransferase 2|ST6GalNAc II|ST6GalNAcII|galNAc alpha-2,6-sialyltransferase II|sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B|sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) B|sialyltransferase 7B|sialyltransferase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ST6GALNAC2, check out the ST6GALNAC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ST6GALNAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ST6GALNAC2 Antibody Picoband™ (A10348)
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