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- Table of Contents
STAR antibody (A00051-1) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Western blot analysis of StAR using anti-StAR antibody (A00051-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
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Anti-StAR Antibody Picoband®
STAR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
STAR antibody was positively detected by WB in:
rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Mouse
Tested
Human
Predicted
Monkey
Predicted
Dog
Predicted
Product Information
Product Name
Anti-StAR Antibody Picoband®
View all STAR Antibodies
View all STAR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human StAR, which shares 85% and 87.5% amino acid (aa) sequence identity with mouse and rat StAR, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
31.9 kDa
Background of STAR
The steroidogenic acute regulatory protein, commonly referred to as StAR (STARD1), is a transport protein. This protein plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. It permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
Cite This Product
Anti-StAR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00051-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
93%
High Homology
Rat
88%
Moderate Homology
Mouse
85%
Moderate Homology
Rabbit
83%
Moderate Homology
Cow
80%
Moderate Homology
Sheep
80%
Moderate Homology
Zebrafish
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for STAR antibody
Publications
Application:
Species:
Effect of Selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters
Published: 2025-12-02
Journal: POULTRY SCIENCE
Application: WB
Impact Factor: 4.20
Species: Rooster
PMID: 41352196
Effects of 3, 4-divanillyltetrahydrofuran from Urtica fissa on sexual dysfunction in diabetic mice
Published: 2022-02-01
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Mouse
PMID: 35121049
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats
Published: 2017-01-12
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.78
Species: Rat
PMID: 28089693
4-Methylcatechol inhibits cell growth and testosterone production in TM3 Leydig cells by reducing mitochondrial activity
Published: 2016-05-05
Journal: ANDROLOGIA
Application: WB
Impact Factor: 1.44
Species: Mouse
PMID: 27147129
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Product Promise
We promise Anti-StAR Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00051-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026