This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
STAT1 antibody (M00036-1) performs well in Flow Cytometry, IP, IHC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
IF analysis of STAT1 using anti-STAT1 antibody (M00036-1).
STAT1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-STAT1 Antibody (M00036-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
STAT1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-STAT1 Antibody (M00036-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-STAT1 Rabbit Monoclonal Antibody
STAT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IP, IHC, WB |
Tested Applications
STAT1 antibody was positively detected by WB in:
human Hela whole cell, human A549 whole cell, human 293T whole cell, human K562 whole cell, mouse heart tissue, mouse brain tissue, human 293T- WT whole cell, human 293T-STAT1 KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
STAT1 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human lung adenocarcinoma tissue, human liver adenocarcinoma tissue, human appendix tissue, mouse bladder tissue, mouse colon tissue, human bladder cancer tissue, human skin cancer tissue, human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-STAT1 Rabbit Monoclonal Antibody
View all STAT1 Antibodies
View all STAT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BEE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
91 kDa
Calculated Molecular Weight
87.3 kDa
Cite This Product
Anti-STAT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036-1)
Protocols
Western Blot protocol for STAT1 antibody
Immunohistochemistry (IHC) protocol for STAT1 antibody
Publications
Application:
Species:
Distinct roles of TREM2 in central nervous system cancers and peripheral cancers
Published: 2024-05-23
Journal: CANCER CELL
Application: WB
Impact Factor: 48.80
Species: Mouse
PMID: 38788719
Pulsatilla chinensis saponins ameliorated murine depression by inhibiting intestinal inflammation mediated IDO1 overexpression and rebalancing tryptophan metabolism
Published: 2023-05-01
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 7.90
Species: Mouse
PMID: 37167824
Review
Have you used Anti-STAT1 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-STAT1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00036-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026