Anti-STAT1 Rabbit Monoclonal Antibody

STAT1 antibody

Boster Bio Anti-STAT1 Rabbit Monoclonal Antibody catalog # M00036-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: M00036-1
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IP, IHC, WB

Product Name

Anti-STAT1 Rabbit Monoclonal Antibody

View all STAT1 Antibodies

SKU/Catalog Number

M00036-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-STAT1 Rabbit Monoclonal Antibody catalog # M00036-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BEE-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human STAT1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00036-1 is reactive to STAT1 in Human, Mouse

Applications

M00036-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

83, 88 kDa

Calculated molecular weight

87.335kDa

Background of STAT1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)

Gene Name

STAT1

Full Name

Signal transducer and activator of transcription 1-alpha/beta

Weight

87.335kDa

Superfamily

transcription factor STAT family

Alternative Names

CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on STAT1, check out the STAT1 Infographic

STAT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00036-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway

Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.

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16 Customer Q&As for Anti-STAT1 Rabbit Monoclonal Antibody

Question

I see that the anti-STAT1 Rabbit Monoclonal antibody M00036-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-05-05

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-STAT1 Rabbit Monoclonal antibody M00036-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-12

Question

Our lab were satisfied with the WB result of your anti-STAT1 Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta testis cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

According to literature, placenta testis does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-11

Question

I am interested in to test anti-STAT1 Rabbit Monoclonal antibody M00036-1 on human b-cell for research purposes, then I may be interested in using anti-STAT1 Rabbit Monoclonal antibody M00036-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

The products we sell, including anti-STAT1 Rabbit Monoclonal antibody M00036-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-30

Question

Would M00036-1 anti-STAT1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

It shows on the product datasheet, M00036-1 anti-STAT1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-09

Question

Would anti-STAT1 Rabbit Monoclonal antibody M00036-1 work for WB with b-cell?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

According to the expression profile of b-cell, STAT1 is highly expressed in b-cell. So, it is likely that anti-STAT1 Rabbit Monoclonal antibody M00036-1 will work for WB with b-cell.

Boster Scientific Support

Answered: 2020-01-07

Question

We have tried in the past anti-STAT1 Rabbit Monoclonal antibody for WB on placenta testis a few years ago. I am using mouse, and I plan to use the antibody for IP next. I am interested in examining placenta testis as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

I took a look at the website and datasheets of our anti-STAT1 Rabbit Monoclonal antibody and it appears that M00036-1 has been tested on mouse in both WB and IP. Thus M00036-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-20

Question

I was wanting to use your anti-STAT1 Rabbit Monoclonal antibody for WB for human b-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell identification?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

You can see on the product datasheet, M00036-1 anti-STAT1 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-13

Question

Is this M00036-1 anti-STAT1 Rabbit Monoclonal antibody reactive to the isotypes of STAT1?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

The immunogen of M00036-1 anti-STAT1 Rabbit Monoclonal antibody is A synthesized peptide derived from human STAT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-25

Question

We have been able to see staining in mouse liver. Are there any suggestions? Is anti-STAT1 Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-05-22

Answer

Based on literature liver does express STAT1. Based on Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-22

Question

See attached the WB image, lot number and protocol we used for b-cell using anti-STAT1 Rabbit Monoclonal antibody M00036-1. Please let me know if you require anything else.

A. Bhatt

Verified customer

Asked: 2018-04-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-23

Question

We need using your anti-STAT1 Rabbit Monoclonal antibody for regulation of interferon-gamma-mediated signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-11-17

Answer

We appreciate your inquiry. This M00036-1 anti-STAT1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-11-17

Question

Is a blocking peptide available for product anti-STAT1 Rabbit Monoclonal antibody (M00036-1)?

P. Li

Verified customer

Asked: 2015-11-12

Answer

We do provide the blocking peptide for product anti-STAT1 Rabbit Monoclonal antibody (M00036-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-11-12

Question

Is there a BSA free version of anti-STAT1 Rabbit Monoclonal antibody M00036-1 available?

L. Baker

Verified customer

Asked: 2015-04-01

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-STAT1 Rabbit Monoclonal antibody M00036-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-04-01

Question

I have a question about product M00036-1, anti-STAT1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Anderson

Verified customer

Asked: 2015-02-12

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00036-1 anti-STAT1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-02-12

Question

We are currently using anti-STAT1 Rabbit Monoclonal antibody M00036-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?

J. Anderson

Verified customer

Asked: 2013-03-06

Answer

The anti-STAT1 Rabbit Monoclonal antibody (M00036-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-03-06

Order DetailsPrice
M00036-1

100μL

$370
M00036-1-30ul

30μL sample

$99
M00036-1-carrier-free

Carrier Free

$370

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M00036-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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