Anti-STAT3 Antibody Picoband™

Boster Bio Anti-STAT3 Antibody Picoband™ catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00007
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-STAT3 Antibody Picoband™

See all STAT3 products

SKU/Catalog Number

A00007

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAT3 Antibody Picoband™ catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00007)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human STAT3 recombinant protein (Position: A2-R215). Human STAT3 shares 100% amino acid (aa) sequence identity with both mouse and rat STAT3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00007 is reactive to STAT3 in Human, Mouse, Rat

Applications

A00007 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For STAT3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P40763

Gene ID

6774

Gene Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Weight

88.068kDa

Superfamily

transcription factor STAT family

Alternative Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3 STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STAT3, check out the STAT3 Infographic

STAT3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for STAT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00007 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
A00007 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500

Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
A00007 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300

Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat

Exposure to Concentrated Ambient Fine Particulate Matter Induces Vascular Endothelial Dysfunction via miR-21

IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway

Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis

Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...

Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...

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15 Customer Q&As for Anti-STAT3 Antibody Picoband™

Question

We want using your anti-STAT3 antibody for nanog activate genes related to proliferation studies. Has this antibody been tested with western blotting on colo320 whole cell lysates? We would like to see some validation images before ordering.

P. Lewis

Verified customer

Asked: 2019-12-12

Answer

We appreciate your inquiry. This A00007 anti-STAT3 antibody is validated on rat brain tissue, mouse brain, testis tissue, colo320 whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-12

Question

Is this A00007 anti-STAT3 antibody reactive to the isotypes of STAT3?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The immunogen of A00007 anti-STAT3 antibody is E.coli-derived human STAT3 recombinant protein (Position: A2-R215). Human STAT3 shares 100% amino acid (aa) sequence identity with both mouse and rat STAT3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-08

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT3 antibody A00007. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-04

Question

We are currently using anti-STAT3 antibody A00007 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-STAT3 antibody (A00007) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

Do you have a BSA free version of anti-STAT3 antibody A00007 available?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT3 antibody A00007 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-05

Question

I see that the anti-STAT3 antibody A00007 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-07

Question

Is a blocking peptide available for product anti-STAT3 antibody (A00007)?

P. Carter

Verified customer

Asked: 2018-12-11

Answer

We do provide the blocking peptide for product anti-STAT3 antibody (A00007). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-11

Question

I was wanting to use your anti-STAT3 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?

L. Carter

Verified customer

Asked: 2018-05-11

Answer

As indicated on the product datasheet, A00007 anti-STAT3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-11

Question

We have observed staining in human upper lobe of lung. Are there any suggestions? Is anti-STAT3 antibody supposed to stain upper lobe of lung positively?

Verified Customer

Verified customer

Asked: 2018-04-04

Answer

Based on literature upper lobe of lung does express STAT3. Based on Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451

Boster Scientific Support

Answered: 2018-04-04

Question

Does anti-STAT3 antibody A00007 work for WB with cervix carcinoma?

M. Carter

Verified customer

Asked: 2017-10-10

Answer

According to the expression profile of cervix carcinoma, STAT3 is highly expressed in cervix carcinoma. So, it is likely that anti-STAT3 antibody A00007 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2017-10-10

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT3 antibody A00007. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-13

Question

I am looking for to test anti-STAT3 antibody A00007 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-STAT3 antibody A00007 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

The products we sell, including anti-STAT3 antibody A00007, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-22

Question

Our lab were satisfied with the WB result of your anti-STAT3 antibody. However we have been able to see positive staining in placenta cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is STAT3 supposed to be expressed?

S. Mangal

Verified customer

Asked: 2017-03-29

Answer

Based on literature, placenta does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451

Boster Scientific Support

Answered: 2017-03-29

Question

I have a question about product A00007, anti-STAT3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Z. Zhang

Verified customer

Asked: 2016-01-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00007 anti-STAT3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-01-07

Question

Would A00007 anti-STAT3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Mitchell

Verified customer

Asked: 2013-11-04

Answer

As indicated on the product datasheet, A00007 anti-STAT3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-11-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00007
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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