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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-STAT3 Antibody Picoband™
Boster Bio Anti-STAT3 Antibody Picoband™ catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00007)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human STAT3 recombinant protein (Position: A2-R215). Human STAT3 shares 100% amino acid (aa) sequence identity with both mouse and rat STAT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00007 is reactive to STAT3 in Human, Mouse, Rat
A00007 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (A00007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # A00007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88KD. The expected band size for STAT3 is at 88KD.
Gene/Protein Information For STAT3 (Source: Uniprot.Org, NCBI)
Signal transducer and activator of transcription 3
transcription factor STAT family
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3 STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on STAT3, check out the STAT3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for STAT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00007 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
A00007 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500
Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
A00007 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300
Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat
Exposure to Concentrated Ambient Fine Particulate Matter Induces Vascular Endothelial Dysfunction via miR-21
IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway
Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment
The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells
Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
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