This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
STAT5A antibody (M01087-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
IHC analysis of STAT5a using anti-STAT5a antibody (M01087-1).
STAT5a was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-STAT5a Antibody (M01087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
STAT5a was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-STAT5a Antibody (M01087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-STAT5a Rabbit Monoclonal Antibody
STAT5A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
STAT5A antibody was positively detected by WB in:
human HEL whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse RAW264.7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
STAT5A antibody was positively detected by IHC in:
human breast cancer tissue, human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
STAT5A antibody was positively detected by IP in:
K562 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-STAT5a Rabbit Monoclonal Antibody
View all STAT5A Antibodies
View all STAT5A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT5a
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
91 kDa
Calculated Molecular Weight
90.6 kDa
Cite This Product
Anti-STAT5a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01087-1)
Protocols
Western Blot protocol for STAT5A antibody
Immunohistochemistry (IHC) protocol for STAT5A antibody
Immunoprecipitation (IP) protocol for STAT5A antibody
Publications
Application:
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
PMID: 23693134
Review
Have you used Anti-STAT5a Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-STAT5a Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01087-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 19, 2026