Anti-STIP1 Antibody Picoband™

Product Name

Anti-STIP1 Antibody Picoband™

View all STI1 Antibodies

SKU/Catalog Number

PB9896

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STIP1 Antibody Picoband™ catalog # PB9896. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STIP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9896)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human STIP1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9896 is reactive to STIP1 in Human, Mouse, Rat

Applications

PB9896 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

62.639kDa

Background of STI1

STIP1 is an adaptor protein that coordinates the functions of HSP70 and HSP90 in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones. The International Radiation Hybrid Mapping Consortium mapped the STIP1 gene to 11q13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of STIP1 using anti-STIP1 antibody (PB9896).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIP1 antigen affinity purified polyclonal antibody (Catalog # PB9896) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIP1 at approximately 63 kDa. The expected band size for STIP1 is at 63 kDa.

Gene/Protein Information For STIP1 (Source: Uniprot.org, NCBI)

Alternative Names

HOP; HOPSTI1L; Hsc70/Hsp90-organizing protein; Hsp70/Hsp90-organizing protein; IEF-SSP-3521; NY-REN-11 antigen; NY-REN-11; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STI1P60; STIP1; stress-induced-phosphoprotein 1; Transformation-sensitive protein IEF SSP 3521 STIP1 HEL-S-94n, HOP, IEF-SSP-3521, P60, STI1, STI1L stress induced phosphoprotein 1 stress-induced-phosphoprotein 1|Hsp70/Hsp90-organizing protein|NY-REN-11 antigen|epididymis secretory sperm binding protein Li 94n|hsc70/Hsp90-organizing protein|renal carcinoma antigen NY-REN-11|transformation-sensitive protein IEF SSP 3521

For more info on STIP1, check out the STIP1 Infographic

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In this infographic, you will see the following information for STIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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