Anti-STMN1 Antibody (C-Term)

Product Name

Anti-STMN1 Antibody (C-Term)

View all Stathmin 1 Antibodies

SKU/Catalog Number

M01194-2

Size

50 µl

Description

Boster Bio Anti-STMN1 Antibody (C-Term) (Catalog # M01194-2). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-STMN1 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01194-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This STMN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 106-140 amino acids from human STMN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01194-2 is reactive to STMN1 in Human, Rat

Applications

M01194-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

17.303kDa

Background of Stathmin 1

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser- 16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-STMN1 Antibody (C-Term) at 1:2000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lane 3: PC-12 whole cell lysate
Lane 4: Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 17 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Overlay histogram showing U-2 OS cells stained with M01194-2(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01194-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For STMN1 (Source: Uniprot.org, NCBI)

Alternative Names

C1orf215; chromosome 1 open reading frame 215; Lag; Leukemia-associated phosphoprotein p18; metablastin; MGC138869; Oncoprotein 18; Op18; OP18MGC138870; phosphoprotein 19; Phosphoprotein p19; PP17; PP19; PR22; prosolin; Protein Pr22; SMNLAP18FLJ32206; stathmin 1; stathmin 1/oncoprotein 18; stathmin; transmembrane protein C1orf215 STMN1 C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN stathmin 1 stathmin|leukemia-associated phosphoprotein p18|metablastin|oncoprotein 18|phosphoprotein 19|phosphoprotein p19|prosolin|stathmin 1/oncoprotein 18|testicular tissue protein Li 189|transmembrane protein C1orf215

For more info on STMN1, check out the STMN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STMN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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