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Product Info Summary
| SKU: | A00053-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-Stromal cell-derived factor 1 CXCL12 Antibody
SKU/Catalog Number
A00053-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Stromal cell-derived factor 1 CXCL12 Antibody (Catalog # A00053-3). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.
Storage & Handling
CXCL12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Stromal cell-derived factor 1 CXCL12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00053-3)
Host
Rabbit
Contents
CXCL12 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CXCL12 antibody was raised against a 16 amino acid peptide near the center of human CXCL12 . The immunogen is located within the last 50 amino acids of CXCL12.
Cross-reactivity
CXCL12 antibody is human and mouse reactive. Multiple isoforms of CXCL12 are known to exist.
Reactive Species
A00053-3 is reactive to CXCL12 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.7 kDa
Background of CXCL12
The CXCL12 protein, also known as SDF1, is a stromal cell-derived alpha chemokine member of the intercrine family. CXCL12 functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4) and CXCR7, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis (reviewed in 1). Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00053-3 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CXCL12 antibody can be used for detection of CXCL12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CXCL12 in HeLa cell lysate with CXCL12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of CXCL12 in HeLa cells with CXCL12 antibody at 5 μg/mL.
Specific Publications For Anti-Stromal cell-derived factor 1 CXCL12 Antibody (A00053-3)
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