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Product Info Summary
| SKU: | A09570-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-STS 1/UBASH3B Antibody
SKU/Catalog Number
A09570-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-STS 1/UBASH3B Antibody catalog # A09570-3. Tested in WB, IHC, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-STS 1/UBASH3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09570-3)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
Peptide
Reactive Species
A09570-3 is reactive to UBASH3B in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
72.7 kDa
Background of UBASH3B
This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09570-3 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunoprecipitation, 1:250-300
ELISA, 1:100-1000
Positive Control
WB: human HEL whole cell lysates, human A549 whole cell lysates, human Caco-2 whole cell lysates, rat lung tissue lysates, mouse lung tissue lysates, mouse spleen tissue lysates
Validation Images & Assay Conditions
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Western blot analysis of STS 1/UBASH3B using anti-STS 1/UBASH3B antibody (A09570-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STS 1/UBASH3B antigen affinity purified polyclonal antibody (A09570-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STS 1/UBASH3B at approximately 70 kDa. The expected band size for STS 1/UBASH3B is at 73 kDa.
Specific Publications For Anti-STS 1/UBASH3B Antibody (A09570-3)
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