Anti-STUB1 Antibody (C-term)

Product Name

Anti-STUB1 Antibody (C-term)

View all CHIP/STUB1 Antibodies

SKU/Catalog Number

M01236-1

Size

50 µl

Description

Boster Bio Anti-STUB1 Antibody (C-term) (Catalog # M01236-1). Tested in WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-STUB1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01236-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1008CT12.4.1

Isotype

IgG1,k

Immunogen

This STUB1 antibody is generated from a mouse immunized with a recombinant protein of human STUB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01236-1 is reactive to STUB1 in Human, Mouse

Reconstitution

Calculated molecular weight

34856 Da

Background of CHIP/STUB1

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01236-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000

Validation Images & Assay Conditions

Gene/Protein Information For STUB1 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase CHIP, 6.3.2.-, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1 {ECO:0000312|HGNC:HGNC:11427}, STUB1 (HGNC:11427) STUB1 CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1 STIP1 homology and U-box containing protein 1 E3 ubiquitin-protein ligase CHIP|CLL-associated KW-8|RING-type E3 ubiquitin transferase CHIP|STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase| NY-CO-7|carboxy terminus of Hsp70-interacting protein|heat shock protein A binding protein 2 (c-terminal)|serologically defined colon cancer 7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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