Anti-STXBP2 Antibody Picoband™

Syntaxin-BP2 antibody

Boster Bio Anti-STXBP2 Antibody Picoband™ catalog # PB9820. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9820
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-STXBP2 Antibody Picoband™

View all Syntaxin-BP2 Antibodies

SKU/Catalog Number

PB9820

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STXBP2 Antibody Picoband™ catalog # PB9820. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STXBP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9820)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human STXBP2, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9820 is reactive to STXBP2 in Human, Mouse, Rat

Applications

PB9820 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

66 kDa

Calculated molecular weight

66.453kDa

Background of Syntaxin-BP2

Syntaxin-binding protein 2, also known as UNC18B, is a protein that in humans is encoded by the STXBP2 gene. The STXBP2 gene is mapped to human chromosome 19p13.3-p13.2 and to the proximal arm of mouse chromosome 8. And this gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Additionally, UNC18B is expressed predominantly as a 2.4-kb message in placenta, lung, liver, kidney, and pancreas, as well as in peripheral blood lymphocytes. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For STXBP2 (Source: Uniprot.org, NCBI)

Gene Name

STXBP2

Full Name

Syntaxin-binding protein 2

Weight

66.453kDa

Superfamily

STXBP/unc-18/SEC1 family

Alternative Names

FHL5; Hunc18b; MUNC18-2; Protein unc-18 homolog 2; Protein unc-18 homolog B; STXBP2; syntaxin binding protein 2; syntaxin-binding protein 2; SyntaxinBP2; Syntaxin-BP2; Unc18-2; UNC18B; unc-18B; UNC18Bpp10122 STXBP2 FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122 syntaxin binding protein 2 syntaxin-binding protein 2|protein unc-18 homolog B|unc-18B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on STXBP2, check out the STXBP2 Infographic

STXBP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STXBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-STXBP2 Antibody Picoband™

Question

Will PB9820 anti-STXBP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

You can see on the product datasheet, PB9820 anti-STXBP2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-01

Question

Does anti-STXBP2 antibody PB9820 work on horse WB with kidney?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

Our lab technicians have not validated anti-STXBP2 antibody PB9820 on horse. You can run a BLAST between horse and the immunogen sequence of anti-STXBP2 antibody PB9820 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-05

Question

We are currently using anti-STXBP2 antibody PB9820 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

The anti-STXBP2 antibody (PB9820) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-04

Question

Is there a BSA free version of anti-STXBP2 antibody PB9820 available?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STXBP2 antibody PB9820 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-01

Question

My question regarding product PB9820, anti-STXBP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-02-19

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9820 anti-STXBP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-02-19

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-STXBP2 antibody PB9820. Let me know if you need anything else.

L. Zhao

Verified customer

Asked: 2017-02-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-02-27

Question

See attached the WB image, lot number and protocol we used for lung using anti-STXBP2 antibody PB9820. Please let me know if you require anything else.

M. Zhang

Verified customer

Asked: 2016-04-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-04-21

Order DetailsPrice
PB9820

100μg

$370
PB9820-10ug

10μg sample (liquid)

$99
PB9820-Biotin

100 μg Biotin conjugated

$570
PB9820-Cy3

100 μg Cy3 conjugated

$570
PB9820-Dylight488

100 μg Dylight488 conjugated

$570
PB9820-Dylight550

100 μg Dylight550 conjugated

$570
PB9820-Dylight594

100 μg Dylight594 conjugated

$570
PB9820-FITC

100 μg FITC conjugated

$570
PB9820-HRP

100 μg HRP conjugated

$570
PB9820-APC

100 μg APC conjugated

$670
PB9820-PE

100 μg PE conjugated

$670
PB9820-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9820
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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