Anti-Sumo SUMO1 Antibody

Product Name

Anti-Sumo SUMO1 Antibody

View all SUMO1 Antibodies

SKU/Catalog Number

A00631

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Sumo SUMO1 Antibody (Catalog # A00631). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Sumo antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Sumo SUMO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00631)

Host

Rabbit

Contents

Sumo Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Sumo antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo. The immunogen is located within the first 50 amino acids of Sumo.

Reactive Species

A00631 is reactive to SUMO1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

11.6 kDa

Background of SUMO1

The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00631 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Sumo antibody can be used for detection of sumo by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SUMO1

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