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- Table of Contents
SUMO1 · Western blot design guide
SUMO1 Western Blot Planning Guide
Plan a SUMO1 Western blot around the catalog-observed 11.6 kDa band, image-backed PA2220 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SUMO1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 11.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 15% | |
| Positive control | Adrenal gland | |
| Negative control | Soft tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 11.6 kDa |
| ⓘ | Localization | Nucleus membrane / Nucleus speckle |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Rat / Mouse / Human |
Section 1
Real Curated SUMO1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adipose tissue |
| Gel % | 15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.2 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2220 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SUMO1 Western Blot Band Size?
Use the product-observed 11.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 11.6 kDa | Matches the authoritative product WB observation. |
| 11.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SUMO1 appearancePlan around 11.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 11.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 11.6 kDa from UniProt P63165; retain as context. |
| Gel selection | 15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2220. |
| Situation | Likely cause | Next action |
|---|---|---|
| 11.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SUMO1 band
PA2220 · SUMO1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SUMO1 Western blot
🧪Use Adrenal gland as the first positive-control candidate and Soft tissue as the HPA Not detected negative candidate.
Positive control: Adrenal gland (High)
Negative control: Soft tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SUMO1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adipose tissue | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Soft tissue | Reported tissue cells | Not detected | Protein (HPA) | HPA → |
Section 3
Advanced SUMO1 Western Blot Tips
Deeper troubleshooting and optimisation questions for SUMO1, answered from its protein features.
Which band should guide the blot?
Use 11.6 kDa, the observation attached to the authoritative PA2220 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 11.6 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Soft tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SUMO1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2220 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
SUMO1 Western Blot Reagents
Rat/Mouse/Human-reactive SUMO1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Sumo 1/SUMO1 Antibody Picoband®
Cat # PA2220
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P63165
- Human Protein Atlas — SUMO1
- PA2220 product record
- PMC3797200 — Estradiol potentiates 8-OH-DPAT-induced sumoylation of 5-HT₁A receptor: characterization and subcellular distribution of sumoylated 5-HT₁A receptors (Psychoneuroendocrinology, 2013)
- PMC7527722 — Klebsiella pneumoniae Reduces SUMOylation To Limit Host Defense Responses (mBio, 2020)
- PMC2034454 — Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation (Nucleic acids research, 2007)