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Product Info Summary
| SKU: | A10695-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Synaptotagmin-11 SYT11 Antibody
SKU/Catalog Number
A10695-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Synaptotagmin-11 SYT11 Antibody catalog # A10695-2. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptotagmin-11 SYT11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10695-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human SYT11(NP_689493.3).
Reactive Species
A10695-2 is reactive to SYT11 in Human, Mouse, Rat
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10695-2 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using SYT11 antibody at 1:670 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 20s.
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Immunofluorescence analysis of mouse brain cells using SYT11 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of rat brain cells using SYT11 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of SH-5Y5Y cells using SYT11 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.
Specific Publications For Anti-Synaptotagmin-11 SYT11 Antibody (A10695-2)
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