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Product Info Summary
| SKU: | A10695-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Synaptotagmin XI SYT11 Antibody
SKU/Catalog Number
A10695-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Synaptotagmin XI SYT11 Antibody catalog # A10695-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptotagmin XI SYT11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10695-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SYT11. AA range:181-230
Reactive Species
A10695-1 is reactive to SYT11 in Human, Mouse, Rat
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10695-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Synaptotagmin XI Polyclonal Antibody
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Western blot analysis of SYT11 Antibody. The lane on the right is blocked with the SYT11 peptide.
Specific Publications For Anti-Synaptotagmin XI SYT11 Antibody (A10695-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Synaptotagmin XI SYT11 Antibody
Question
Here is the WB image, lot number and protocol we used for amygdala using anti-Synaptotagmin XI antibody A10695-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-05
Question
We are currently using anti-Synaptotagmin XI antibody A10695-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-Synaptotagmin XI antibody (A10695-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
I was wanting to use your anti-Synaptotagmin XI antibody for WB for rat amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat amygdala identification?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It shows on the product datasheet, A10695-1 anti-Synaptotagmin XI antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-28
Question
I see that the anti-Synaptotagmin XI antibody A10695-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-24
Question
I have a question about product A10695-1, anti-Synaptotagmin XI antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10695-1 anti-Synaptotagmin XI antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
Does A10695-1 anti-Synaptotagmin XI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
You can see on the product datasheet, A10695-1 anti-Synaptotagmin XI antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-30
Question
Is this A10695-1 anti-Synaptotagmin XI antibody reactive to the isotypes of SYT11?
G. Mitchell
Verified customer
Asked: 2017-02-14
Answer
The immunogen of A10695-1 anti-Synaptotagmin XI antibody is Synthesized peptide derived from the Internal region of human Synaptotagmin XI. at AA rangle: 150-230. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-02-14
