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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Syntenin/SDCBP Antibody Picoband™
Boster Bio Anti-Syntenin/SDCBP Antibody Picoband™ catalog # A02475-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Syntenin/SDCBP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02475-2)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human Syntenin/SDCBP recombinant protein (Position: S2-S259).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins.
A02475-2 is reactive to SDCBP in Human, Mouse, Rat
A02475-2 is guaranteed for ELISA, WB Boster Guarantee
Background of Syntenin 1
Syntenin-1 is a protein that in humans is encoded by the SDCBP gene. It is mapped to 8q12.1. The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. Related pseudogenes have been identified on multiple chromosomes.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SDCBP using anti-SDCBP antibody (A02475-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCBP antigen affinity purified polyclonal antibody (Catalog # A02475-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCBP at approximately 32KD. The expected band size for SDCBP is at 32KD.
Gene/Protein Information For SDCBP (Source: Uniprot.org, NCBI)
MDA9; MDA-9; melanoma differentiation associated protein-9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; ST1; SYCLTACIP18; syndecan binding protein (syntenin); Syndecan-binding protein 1; syntenin-1 SDCBP MDA-9, MDA9, ST1, SYCL, TACIP18 syndecan binding protein syntenin-1|melanoma differentiation associated protein-9|pro-TGF-alpha cytoplasmic domain-interacting protein 18|scaffold protein Pbp1|syndecan binding protein (syntenin)|syndecan-binding protein 1*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on SDCBP, check out the SDCBP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDCBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for A02475-2
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