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Product Info Summary
| SKU: | M05511 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-TAF4 Antibody (C-Term)
SKU/Catalog Number
M05511
Size
50 µl
Description
Boster Bio Anti-TAF4 Antibody (C-Term) (Catalog # M05511). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TAF4 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05511)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TAF4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1021-1062 amino acids from human TAF4.
Reactive Species
M05511 is reactive to TAF4 in Human
Calculated molecular weight
110.1 kDa
Background of TAF4
Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05511 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-TAF4 Antibody (C-Term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: CCRF-CEM whole cell lysate
Lane 6: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 110 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M05511 staining TAF4 in human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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M05511 staining TAF4 in human colon tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-TAF4 Antibody (C-Term) (M05511)
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