Anti-TAF9 Antibody

Product Name

Anti-TAF9 Antibody

View all TAF9 Antibodies

SKU/Catalog Number

A05319

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TAF9 Antibody (Catalog # A05319). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TAF9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-TAF9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05319)

Host

Rabbit

Contents

TAF9 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9. The immunogen is located within amino acids 110 - 160 of TAF9.

Cross-reactivity

TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.

Reactive Species

A05319 is reactive to TAF9 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.0 kDa

Background of TAF9

Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05319 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TAF9

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