Product Info Summary
| SKU: | PA2135 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Tafazzin/TAZ Antibody Picoband®
SKU/Catalog Number
PA2135
BA3118-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tafazzin/TAZ Antibody catalog # PA2135. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tafazzin/TAZ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2135)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Tafazzin, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2135 is reactive to TAZ in Mouse, Rat
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.5 kDa
Background of TAZ
Tafazzin is a protein that in humans is encoded by the TAFAZZIN gene. This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2135 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat heart tissue, rat skeletal muscle tissue, rat kidney tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TAZ using anti-TAZ antibody (PA2135).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAZ antigen affinity purified polyclonal antibody (PA2135) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAZ at approximately 33 kDa. The expected band size for TAZ is at 33 kDa.
Specific Publications For Anti-Tafazzin/TAZ Antibody Picoband® (PA2135)
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4 Customer Q&As for Anti-Tafazzin/TAZ Antibody Picoband®
Question
We are currently using anti-Tafazzin/TAZ antibody PA2135 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The anti-Tafazzin/TAZ antibody (PA2135) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18
Question
We have observed staining in mouse small intestine peyer's patch. Any tips? Is anti-Tafazzin/TAZ antibody supposed to stain small intestine peyer's patch positively?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
From literature small intestine peyer's patch does express TAZ. From Uniprot.org, TAZ is expressed in small intestine peyer's patch, heart skeletal muscle, among other tissues. Regarding which tissues have TAZ expression, here are a few articles citing expression in various tissues:
Heart, and Skeletal muscle, Pubmed ID: 8630491
Boster Scientific Support
Answered: 2019-07-03
Question
Our team were content with the WB result of your anti-Tafazzin/TAZ antibody. However we have seen positive staining in small intestine peyer's patch isoform 1: membrane using this antibody. Is that expected? Could you tell me where is TAZ supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
According to literature, small intestine peyer's patch does express TAZ. Generally TAZ expresses in isoform 1: membrane. Regarding which tissues have TAZ expression, here are a few articles citing expression in various tissues:
Heart, and Skeletal muscle, Pubmed ID: 8630491
Boster Scientific Support
Answered: 2019-06-17
Question
you antibody using your anti-Tafazzin/TAZ antibody for mitochondrial respiratory chain complex i assembly studies. Has this antibody been tested with western blotting on rat heart tissue? We would like to see some validation images before ordering.
F. Carter
Verified customer
Asked: 2015-02-12
Answer
Thanks for your inquiry. This PA2135 anti-Tafazzin/TAZ antibody is validated on rat heart tissue, skeletal muscle tissue, tissue lysate, liver tissue, hela cell lysate, smmc cell lysate, scg cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-02-12


