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Product Info Summary
| SKU: | A00933 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Tafazzin/TAZ Antibody Picoband®
SKU/Catalog Number
A00933
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tafazzin/TAZ Antibody Picoband® catalog # A00933. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tafazzin/TAZ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00933)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tafazzin/TAZ recombinant protein (Position: M73-R292).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00933 is reactive to TAZ in Human
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.5 kDa
Background of TAZ
Tafazzin is a protein that in humans is encoded by the TAFAZZIN gene. This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00933 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human A549 whole cell
IHC: human colon cancer
Validation Images & Assay Conditions
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Western blot analysis of TAZ using anti-TAZ antibody (A00933).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAZ antigen affinity purified polyclonal antibody (A00933) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAZ at approximately 33 kDa. The expected band size for TAZ is at 33 kDa.
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IHC analysis of TAZ using anti-TAZ antibody (A00933).
TAZ was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAZ Antibody (A00933) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Tafazzin/TAZ Antibody Picoband® (A00933)
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5 Customer Q&As for Anti-Tafazzin/TAZ Antibody Picoband®
Question
We ordered your anti-Tafazzin/TAZ antibody for ELISA on small intestine peyer's patch in a previous project. I am using rat, and I plan to use the antibody for WB next. We want examining small intestine peyer's patch as well as heart skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
I have checked the website and datasheets of our anti-Tafazzin/TAZ antibody and I see that A00933 has been tested on rat in both ELISA and WB. Thus A00933 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-18
Question
I am interested in using your anti-Tafazzin/TAZ antibody for mitochondrial protein import studies. Has this antibody been tested with western blotting on rat skeletal muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate your inquiry. This A00933 anti-Tafazzin/TAZ antibody is tested on rat skeletal muscle tissue, mouse skeletal muscle tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-09
Question
Our lab were satisfied with the WB result of your anti-Tafazzin/TAZ antibody . However we have been able to see positive staining in heart skeletal muscle isoform 1: membrane using this antibody. Is that expected? Could you tell me where is TAZ supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Based on literature, heart skeletal muscle does express TAZ. Generally TAZ expresses in isoform 1: membrane. Regarding which tissues have TAZ expression, here are a few articles citing expression in various tissues:
Heart, and Skeletal muscle, Pubmed ID: 8630491
Boster Scientific Support
Answered: 2019-08-15
Question
We have been able to see staining in mouse heart skeletal muscle. Are there any suggestions? Is anti-Tafazzin/TAZ antibody supposed to stain heart skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
Based on literature heart skeletal muscle does express TAZ. Based on Uniprot.org, TAZ is expressed in small intestine peyer's patch, heart skeletal muscle, among other tissues. Regarding which tissues have TAZ expression, here are a few articles citing expression in various tissues:
Heart, and Skeletal muscle, Pubmed ID: 8630491
Boster Scientific Support
Answered: 2018-06-13
Question
We are currently using anti-Tafazzin/TAZ antibody A00933 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
T. Moore
Verified customer
Asked: 2017-03-30
Answer
The anti-Tafazzin/TAZ antibody (A00933) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-30
