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- Table of Contents
TAGLN antibody (A03962-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of HepG2 cells using anti-TAGLN/Transgelin antibody (A03962-2).
Overlay histogram showing HepG2 cells stained with A03962-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAGLN/Transgelin Antibody (A03962-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with A03962-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAGLN/Transgelin Antibody (A03962-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TAGLN/Transgelin Antibody Picoband®
TAGLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TAGLN antibody was positively detected by WB in:
human HepG2 whole cell, rat stomach tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TAGLN antibody was positively detected by IHC in:
human esophageal squamous carcinoma tissue, human lung cancer tissue, human lymphoma tissue, human adrenal cortical adenocarcinoma tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TAGLN antibody was positively detected by ICC/IF in:
CACO-2 cell
TAGLN antibody was positively detected by IF in:
mouse heart tissue, rat heart tissue, human esophagus cancer tissue
TAGLN antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Recommend
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TAGLN/Transgelin Antibody Picoband®
View all TAGLN Antibodies
View all TAGLN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TAGLN/Transgelin recombinant protein (Position: Q15-E170).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
22.6 kDa
Background of TAGLN
Transgelin, also known as SM22 alpha, is a protein that in humans is encoded by the TAGLN gene. This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization.
Cite This Product
Anti-TAGLN/Transgelin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03962-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
98%
High Homology
Sheep
98%
High Homology
Cow
97%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Zebrafish
68%
Low homology
Drosophila
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TAGLN antibody
Immunohistochemistry (IHC) protocol for TAGLN antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TAGLN antibody
Immunofluorescence (IF) protocol for TAGLN antibody
Flow Cytometry (FC) protocol for TAGLN antibody
Publications
Application:
Species:
Altered TAGLN Expression During HBV Replication Correlates with OPA1 Levels
Published: 2026-08-17
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB,IHC
Impact Factor: 5.60
Species: Mouse,Human
PMID: 42653342
PTPRE Exacerbates Phenotypic Transformation of Vascular Smooth Muscle Cells and Vasomotor Dysfunction in Salt-Sensitive Hypertension
Published: 2026-01-06
Journal: CELL BIOLOGY INTERNATIONAL
Application: WB,IHC
Impact Factor: 3.10
Species: Mouse
PMID: 41492999
Integrative proteomics and metabolomics explore the effect and mechanism of Qiyin granules on improving nonalcoholic fatty liver disease
Published: 2024-02-24
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Rat
PMID: 38444462
The expression and significance of IL-6, IFN-γ, SM22α, and MMP-2 in rat model of aortic dissection.
Published: 2017-02-01
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 1.78
Species: Rat
PMID: 28239811
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Product Promise
We promise Anti-TAGLN/Transgelin Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03962-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026