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Product Info Summary
| SKU: | M02859 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2)
SKU/Catalog Number
M02859
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2) catalog # M02859. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02859)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
2G10D2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Talin 1/TLN1 recombinant protein (Position: Q1658-N1876).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02859 is reactive to TLN1 in Human, Mouse, Rat
Observed Molecular Weight
250-270 kDa
Calculated molecular weight
269.8 kDa
Background of TLN1
Talin-1 is a protein that in humans is encoded by the TLN1 gene. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02859 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Raji whole cell, human SW620 whole cell, human COLO320 whole cell, human 293T whole cell, rat lung tissue, mouse lung tissue, mouse liver tissue
FCM: JK cell
Validation Images & Assay Conditions
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Western blot analysis of TLN1 using anti-TLN1 antibody (M02859).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TLN1 antigen affinity purified monoclonal antibody (Catalog # M02859) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TLN1 at approximately 250-270 kDa. The expected band size for TLN1 is at 270 kDa.
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Flow Cytometry analysis of JK cells using anti-TLN1 antibody (M02859).
Overlay histogram showing JK cells stained with M02859 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TLN1 Antibody (M02859, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2) (M02859)
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