Anti-Tamalin GRASP Antibody

Product Name

Anti-Tamalin GRASP Antibody

View all Grasp Antibodies

SKU/Catalog Number

A11078

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Tamalin GRASP Antibody (Catalog # A11078). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Tamalin GRASP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11078)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11078 is reactive to Grasp in Human, Mouse

Applications

A11078 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.337kDa

Background of Grasp

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs).  The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs.  The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins.  It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:20,000
IP: 1μg
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of Tamalin expression in non-transfected control (lane 1) and HEK293 cells (lane 2). Tamalin was detected using rabbit anti-Tamalin Antigen Affinity purified polyclonal antibody (Catalog # A11078) at 1:2000. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

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Mouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.

Gene/Protein Information For Grasp (Source: Uniprot.org, NCBI)

Alternative Names

GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein; MNCb-4428; Tamalin Tamalin|Grasp, ta|trafficking regulator and scaffold protein tamalin|general receptor for phosphoinositides 1-associated scaffold protein|GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein|GRP1-associated scaffold protein

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In this infographic, you will see the following information for Grasp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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