Anti-Tapasin TAPBP Antibody

Product Name

Anti-Tapasin TAPBP Antibody

View all Tapasin Antibodies

SKU/Catalog Number

A04714

Size

200μg/vial

Form

Liquid

Description

Boster Bio Anti-Tapasin TAPBP Antibody (Catalog# A04714). Tested in IP, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tapasin TAPBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04714)

Host

Rabbit

Contents

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to aa 2-20 of human tapasin with a cysteine residue added

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with bovine Tapasin.

Reactive Species

A04714 is reactive to TAPBP in Human

Applications

A04714 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

47.626kDa

Background of Tapasin

EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric and ECL). Detects a band of ~48kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Western blot analysis of Tapasin expression in MW marker (lane 1) and A431 whole cell lysates (lane 2). Tapasinat 48KD was detected using rabbit anti-Tapasin Antigen Affinity purified polyclonal antibody (Catalog # A04714) at 1: 1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For TAPBP (Source: Uniprot.org, NCBI)

Alternative Names

NGS17; NGS-17; TAP binding protein (tapasin); TAP Binding Protein; TAPA; Tapasin; TAPATAP-associated protein; TAPBP; TPN; TPSN; TPSNTAP-binding protein TAPBP NGS17, TAPA, TPN, TPSN TAP binding protein tapasin|NGS-17|TAP binding protein (tapasin)|TAP-associated protein

For more info on TAPBP, check out the TAPBP Infographic

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In this infographic, you will see the following information for TAPBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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