Anti-TATA Box-binding Protein Associated Factor 15-TAF15 Monoclonal Antibody

Product Name

Anti-TATA Box-binding Protein Associated Factor 15-TAF15 Monoclonal Antibody

View all TAF15 Antibodies

SKU/Catalog Number

M03567

Size

100ul

Description

Boster Bio Anti-TATA Box-binding Protein Associated Factor 15-TAF15 Monoclonal Antibody catalog # M03567. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TATA Box-binding Protein Associated Factor 15-TAF15 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03567)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Immunogen

Full length recombinant human TAF15 expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03567 is reactive to TAF15 in Human, Mouse, Rat

Applications

M03567 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

61.83kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1,000. IF/ICC: 1:500-1:1,000

Validation Images & Assay Conditions

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Western blot analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, M03567, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.
Double band at 56 and 68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.

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Immunofluorescent analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, M03567, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The M03567 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.

Gene/Protein Information For TAF15 (Source: Uniprot.org, NCBI)

Alternative Names

68kDa; hTAFII68; Npl3; RBP56TAFII68; RNA-binding protein 56; TAF(II)68; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2NRBP56/CSMF fusion; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TBP-associated factor 15,68 kDa TATA-binding protein-associated factor TAF15 Npl3, RBP56, TAF2N, TAFII68 TATA-box binding protein associated factor 15 TATA-binding protein-associated factor 2N|RBP56/CSMF fusion|TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa|TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56)|TATA box-binding protein-associated factor 2N (RNA-binding protein 56)|TBP-associated factor 15

For more info on TAF15, check out the TAF15 Infographic

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In this infographic, you will see the following information for TAF15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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