|Validated Species:||Bovine, Human|
Data & Images
|Product Name||Anti-Tau Antibody (Monoclonal, TAU-2)|
|Description||Mouse IgG monoclonal antibody for Tau, microtubule-associated protein tau (MAPT) detection. Tested with WB, IHC-P in Human;bovine. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tau Antibody (Monoclonal, TAU-2) TAU-2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1093)|
|Replacement Item||This antibody may replace the following items: sc-101556|sc-101555|sc-28976|sc-19546|sc-19547 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Bovine microtubule-associated proteins(MAPs).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Microtubule-associated protein tau|
|Molecular Weight||78564 MW|
|Protein Function||Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.|
|Tissue Specificity||Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited areas of the CNS.|
|Sequence Similarities||Contains 4 Tau/MAP repeats.|
|Subcellular Localization||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.|
|Alternative Names||Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;Mapt;Mtapt, Tau;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|tangles & tau| signal transduction|cytoskeleton / ecm|cytoskeleton|microtubules|mt associated proteins| neuroscience|cell type marker|neuron marker|axon marker||
Background for Microtubule-associated protein tau
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Tau Antibody (Monoclonal, TAU-2) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, bovine, By Heat
Western blot, 0.5-1μg/ml, Human, bovine
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Tau Antibody (Monoclonal, TAU-2) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate