SKU MA1093
Size 100μg/vial
Reactivity Bovine, Human
Clonality Monoclonal
Clone Number TAU-2
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Tau Antibody (Monoclonal, TAU-2)
SKU/Catalog Number MA1093
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Tau, microtubule-associated protein tau (MAPT) detection. Tested with WB, IHC-P in Human;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Tau Antibody (Monoclonal, TAU-2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1093)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Bovine microtubule-associated proteins(MAPs).
Reactivity Bovine, Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, bovine, By Heat
Western blot, 0.5-1μg/ml, Human, bovine

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1093 1 WB anti tau antibody monoclonal tau 2.jpg

Anti-Tau antibody (monoclonal), MA1093, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P19332
Gene Name MAPT
Protein Name Microtubule-associated protein tau
Tissue Specificity Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited areas of the CNS.
Alternative Names Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;Mapt;Mtapt, Tau;
Subcellular Localization Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Molecular Weight 78564 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Research Areas Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction

*You can search these to find other products in these research areas.
Background The microtubule-associated proteins(MAPs) coassemble with tubulin into microtubules in vitro. Microtubule-associated protein tau appears to be enriched in axons. Tau are composed of 352 to 441 amino acids. The isoforms differ from each other by the presence or absence of 29-amino acid or 58-amino acid inserts located in the N terminus and a 31-amino repeat located in the C terminus. tau is important in establishing and maintaining neuronal morphology and is a major component of the neurofibrillary tangles(NFTs) characteristic of Alzheimer's brain. Microtubule-associated protein tau(MTBT1) is mapped to chromosome 17q21.

Other Recommended Resources

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Order Product (MA1093)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.

Troubleshooting

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We promise all of our products perform as described in datasheets.

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Publications

Micro-RNA-137 Inhibits Tau Hyperphosphorylation in Alzheimer's Disease and Targets the CACNA1C Gene in Transgenic Mice and Human Neuroblastoma %u2026
Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment
The expression of insulin-like growth factor-1 in senior patients with diabetes and dementia
Panax notoginsenoside Rb1 ameliorates Alzheimer?s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression
Chen C, Gu J, Basurto-Islas G, Jin N, Wu F, Gong CX, Iqbal K, Liu F. Sci Rep. 2017 Oct 18;7(1):13478. doi: 10.1038/s41598-017-13791-5. Up-regulation of casein kinase 1ε is involved in tau pathogenesis in Alzheimer’s disease
Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.
Zhu Yf, Li Xh, Yuan Zp, Li Cy, Tian Rb, Jia W, Xiao Zp. Eur J Pharmacol. 2015 Sep 5;762:239-46. Doi: 10.1016/J.Ejphar.2015.06.002. Epub 2015 Jun 3. Allicin Improves Endoplasmic Reticulum Stress-Related Cognitive Deficits Via Perk/Nrf2 Antioxidativ...
Tan Wf, Cao Xz, Wang Jk, Lv Hw, Wu By, Ma H. Clin Exp Pharmacol Physiol. 2010 Oct;37(10):1010-5. Doi: 10.1111/J.1440-1681.2010.05433.X. Protective Effects Of Lithium Treatment For Spatial Memory Deficits Induced By Tau Hyperphosphorylation In Sple...
Tu Q, Pang L, Chen Y, Zhang Y, Zhang R, Lu B, Wang J. Analyst. 2014 Jan 7;139(1):105-15. Doi: 10.1039/C3An01796F. Epub 2013 Oct 25. Effects Of Surface Charges Of Graphene Oxide On Neuronal Outgrowth And Branching.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tau antibody, phf tau antibody, phf-tau antibody