Click image to see more details
Product Info Summary
| SKU: | M00097-11 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21)
SKU/Catalog Number
M00097-11
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21) catalog # M00097-11. Tested in WB, IHC, IF, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00097-11)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
32M21
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser396 of human Tau.
Reactive Species
M00097-11 is reactive to MAPT in Human, Mouse, Rat
Background of MAPT
MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00097-11 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 1:500-2000 | |
| Immunohistochemistry | 1:50-200 | |
| Immunofluorescence | 1:50-200 | |
| Immunocytochemistry/Immunofluorescence | 1:50-200 |
Validation Images & Assay Conditions
Click image to see more details
IF analysis of TAU/MAPT using anti-TAU/MAPT antibody (M00097-11) and anti-Alpha Tubulin antibody (M03989-3).
TAU/MAPT was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TAU/MAPT Antibody (M00097-11) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21) (M00097-11)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-TAU/MAPT (Phospho-S396) Antibody (Monoclonal, 32M21)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
