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Product Info Summary
| SKU: | M00431 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TCF7L2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00431
BM4559 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TCF7L2 Rabbit Monoclonal Antibody catalog # M00431. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCF7L2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00431)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FIB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TCF7L2
Reactive Species
M00431 is reactive to TCF7L2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00431 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: Jurkat cell
IHC: human colonic cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of TCF7L2 expression in Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colonic cancer, using TCF7L2 Antibody.
Specific Publications For Anti-TCF7L2 Rabbit Monoclonal Antibody (M00431)
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Customer Q&As
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4 Customer Q&As for Anti-TCF7L2 Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-TCF7L2 Rabbit Monoclonal antibody M00431 available?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCF7L2 Rabbit Monoclonal antibody M00431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-30
Question
I would like to test anti-TCF7L2 Rabbit Monoclonal antibody M00431 on human liver for research purposes, then I may be interested in using anti-TCF7L2 Rabbit Monoclonal antibody M00431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The products we sell, including anti-TCF7L2 Rabbit Monoclonal antibody M00431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-24
Question
Will anti-TCF7L2 Rabbit Monoclonal antibody M00431 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of liver, TCF7L2 is highly expressed in liver. So, it is likely that anti-TCF7L2 Rabbit Monoclonal antibody M00431 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-TCF7L2 Rabbit Monoclonal antibody M00431 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The anti-TCF7L2 Rabbit Monoclonal antibody (M00431) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-17
