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- Table of Contents
TDRD3 antibody (A08978) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of TDRD3 using anti-TDRD3 antibody (A08978)
TDRD3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
TDRD3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot for TDRD3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the TDRD3 Western Blot GuideBundle Save
Anti-TDRD3 Antibody Picoband®
TDRD3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
TDRD3 antibody was positively detected by WB in:
human placenta tissue, human HL-60 whole cell, human Hela whole cell, human PC-3 whole cell, human HEK293 whole cell, human A549 whole cell, human A431 whole cell, rat brain tissues, rat liver tissue, mouse brain tissue, mouse lung tissue, mouse liver tissue, mouse NIH3T3 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TDRD3 antibody was positively detected by IHC in:
human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TDRD3 antibody was positively detected by IF in:
human intestinal cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunofluorescence
2μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TDRD3 Antibody Picoband®
View all TDRD3 Antibodies
View all TDRD3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TDRD3, which shares 96.3% and 100% amino acid (aa) sequence identity with mouse and rat TDRD3, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65-73 kDa
Calculated Molecular Weight
73.2 kDa
Background of TDRD3
Tudor domain-containing protein 3 is a protein that in humans is encoded by the TDRD3 gene. This gene is mapped to 13q21.2. It contains a Tudor domain and UBA protein domain and has three distinct Protein isoforms. TDRD3 is a multifunctional protein that acts as a transcriptional coactivator in the nucleus and as a scaffolding protein in cytoplasmic stress granules.
Cite This Product
Anti-TDRD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08978)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Sheep
96%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TDRD3 antibody
Immunohistochemistry (IHC) protocol for TDRD3 antibody
Immunofluorescence (IF) protocol for TDRD3 antibody
Publications
Application:
Species:
Maternal cafeteria diet intake influences offspring development in Wistar rats: a pilot study
Published: 2026-07-25
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.90
Species: Rat
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Product Promise
We promise Anti-TDRD3 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A08978
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026