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Product Info Summary
| SKU: | M05546 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TDT DNTT Monoclonal Antibody
SKU/Catalog Number
M05546
BM5768 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TDT DNTT Monoclonal Antibody catalog # M05546. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TDT DNTT Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05546)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHA-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TDT Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Reactive Species
M05546 is reactive to DNTT in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
58.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05546 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human Jurkat whole cell, human MOLT-4 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TDT using anti-TDT antibody (M05546).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDT antigen affinity purified monoclonal antibody (M05546) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDT at approximately 59 kDa. The expected band size for TDT is at 59 kDa.
Specific Publications For Anti-TDT DNTT Monoclonal Antibody (M05546)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TDT DNTT Monoclonal Antibody
Question
I was wanting to use your anti-TDT Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
As indicated on the product datasheet, M05546 anti-TDT Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-TDT Monoclonal antibody M05546 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The anti-TDT Monoclonal antibody (M05546) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01
Question
We are interested in to test anti-TDT Monoclonal antibody M05546 on human bone marrow for research purposes, then I may be interested in using anti-TDT Monoclonal antibody M05546 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The products we sell, including anti-TDT Monoclonal antibody M05546, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-10
Question
Is this M05546 anti-TDT Monoclonal antibody reactive to the isotypes of DNTT?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The immunogen of M05546 anti-TDT Monoclonal antibody is A synthesized peptide derived from human TDT Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
I have attached the WB image, lot number and protocol we used for bone marrow using anti-TDT Monoclonal antibody M05546. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-TDT Monoclonal antibody M05546. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
My team were happy with the WB result of your anti-TDT Monoclonal antibody. However we have observed positive staining in testis nucleus. using this antibody. Is that expected? Could you tell me where is DNTT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
From literature, testis does express DNTT. Generally DNTT expresses in nucleus. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2019-07-05
Question
Will M05546 anti-TDT Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
As indicated on the product datasheet, M05546 anti-TDT Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-03
Question
I am interested in using your anti-TDT Monoclonal antibody for dna metabolic process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
Thanks for your inquiry. This M05546 anti-TDT Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-25
Question
Do you have a BSA free version of anti-TDT Monoclonal antibody M05546 available?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TDT Monoclonal antibody M05546 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-05
Question
We have observed staining in human thymus. Are there any suggestions? Is anti-TDT Monoclonal antibody supposed to stain thymus positively?
W. Zhang
Verified customer
Asked: 2018-10-24
Answer
Based on literature thymus does express DNTT. Based on Uniprot.org, DNTT is expressed in bone marrow, thymus, testis, among other tissues. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2018-10-24
Question
I have a question about product M05546, anti-TDT Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05546 anti-TDT Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-18
Question
Will anti-TDT Monoclonal antibody M05546 work for WB with bone marrow?
D. Patel
Verified customer
Asked: 2015-11-18
Answer
According to the expression profile of bone marrow, DNTT is highly expressed in bone marrow. So, it is likely that anti-TDT Monoclonal antibody M05546 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2015-11-18
Question
I see that the anti-TDT Monoclonal antibody M05546 works with WB, what is the protocol used to produce the result images on the product page?
O. Anderson
Verified customer
Asked: 2014-10-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-10-16
Question
Is a blocking peptide available for product anti-TDT Monoclonal antibody (M05546)?
P. Jones
Verified customer
Asked: 2014-02-11
Answer
We do provide the blocking peptide for product anti-TDT Monoclonal antibody (M05546). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-02-11
