This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
DNTT antibody (PB9162) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
Filter:
Western blot analysis of TdT using anti-TdT antibody (PB9162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TDT protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TDT protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
Bundle Save
Anti-TdT/DNTT Antibody Picoband®
DNTT antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
DNTT antibody was positively detected by WB in:
human Jurkat whole cel
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
Human
Tested
Monkey
Predicted
Dog
Predicted
Bovine
Predicted
Product Information
Product Name
Anti-TdT/DNTT Antibody Picoband®
View all DNTT Antibodies
View all DNTT Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
58.5 kDa
Background of DNTT
Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
Cite This Product
Anti-TdT/DNTT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9162)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
91%
High Homology
Cow
90%
High Homology
Sheep
89%
Moderate Homology
Rabbit
86%
Moderate Homology
Rat
84%
Moderate Homology
Mouse
81%
Moderate Homology
Zebrafish
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DNTT antibody
Publications
Application:
Jiang Y, Yu M, Hu X, Han L, Yang K, Ba H, Zhang Z, Yin B, Yang XP, Li Z, Wang J. Cell Death Differ. 2017 Apr;24(4):660-671. doi: 10.1038/cdd.2016.162. Epub 2017 Feb 10. STAT1 mediates transmembrane TNF-alpha-induced formation of death-inducing sig...
PMID: 28186502
Zhang R, Pei H, Ru L, Li H, Liu G. Biomed Rep. 2013 Nov;1(6):895-900. Epub 2013 Sep 6. Bone Morphogenetic Protein 7 Upregulates The Expression Of Nestin And Glial Fibrillary Acidic Protein In Rats With Cerebral Ischemia-Reperfusion Injury.
PMID: 24649049
Xu Bb, Liu Cq, Gao X, Zhang Wq, Wang Sw, Cao Yl. J Asian Nat Prod Res. 2005 Jun;7(3):215-24. Possible Mechanisms Of The Protection Of Ginsenoside Re Against Mptp-Induced Apoptosis In Substantia Nigra Neurons Of Parkinson'S Disease Mouse Model.
PMID: 15621629
Gong Wh, Zheng Wx, Wang J, Chen Sh, Pang B, Hu Xm, Cao Xl. World J Gastroenterol. 2012 Sep 21;18(35):4934-43. Coexistence Of Hyperlipidemia And Acute Cerebral Ischemia/Reperfusion Induces Severe Liver Damage In A Rat Model.
PMID: 23002367
Zeng C, Ke Z, Song Y, Yao Y, Hu X, Zhang M, Li H, Yin J. Exp Mol Pathol. 2013 Aug;95(1):23-31. Doi: 10.1016/J.Yexmp.2013.04.002. Epub 2013 Apr 28. Annexin A3 Is Associated With A Poor Prognosis In Breast Cancer And Participates In The Modulation O...
PMID: 23631820
Xi D, Wang M, Ye H, Luo X, Ning Q. Plos One. 2013 Nov 26;8(11):E82330. Doi: 10.1371/Journal.Pone.0082330. Ecollection 2013. Combined Adenovirus-Mediated Artificial Micrornas Targeting Mfgl2, Mfas, And Mtnfr1 Protect Against Fulminant Hepatic Failu...
PMID: 24303082
Yang Q, Zhang F, Ding Y, Huang J, Chen S, Wu Q, Wang Z, Wang Z, Chen C. Br J Cancer. 2014 Mar 4;110(5):1288-97. Doi: 10.1038/Bjc.2014.1. Epub 2014 Jan 21. Antitumour Activity Of The Recombination Polypeptide Gst-Nt21Mp Is Mediated By Inhibition Of...
PMID: 24448360
Luo T, Xia Z, Ansley Dm, Ouyang J, Granville Dj, Li Y, Xia Zy, Zhou Qs, Liu Xy. Anesth Analg. 2005 Jun;100(6):1653-9. Propofol Dose-Dependently Reduces Tumor Necrosis Factor-Alpha-Induced Human Umbilical Vein Endothelial Cell Apoptosis: Effects On...
PMID: 15920191
Luo T, Xia Z. Anesth Analg. 2006 Jul;103(1):110-6, Table Of Contents. A Small Dose Of Hydrogen Peroxide Enhances Tumor Necrosis Factor-Alpha Toxicity In Inducing Human Vascular Endothelial Cell Apoptosis: Reversal With Propofol.
PMID: 16790636
Tochinai R, Ando M, Suzuki T, Suzuki K, Nagata Y, Hata C, Uchida K, Kobayashi T, Kado S, Kaneko K. Exp Toxicol Pathol. 2013 Sep;65(6):737-43. Doi: 10.1016/J.Etp.2012.09.008. Epub 2012 Nov 4. Histopathological Studies Of Microtubule Disassembling A...
PMID: 23131582
Review
Have you used Anti-TdT/DNTT Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-TdT/DNTT Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9162
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026