Product Info Summary
SKU: | A01031 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Tec Antibody Picoband™
SKU/Catalog Number
A01031
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tec Antibody Picoband™ catalog # A01031. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tec Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01031)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Tec, which shares 97.5% amino acid (aa) sequence identity with both mouse and rat Tec.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01031 is reactive to TEC in Human, Mouse, Rat
Applications
A01031 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
73 kDa
Calculated molecular weight
73.581kDa
Background of TEC
TEC (TEC Protein Tyrosine Kinase) is an enzyme that in humans is encoded by the TEC gene. The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. By fluorescence in situ hybridization, Sato et al. (1994) mapped the gene to 4p12, the same location reported for TXK. Mouse Tec is a non-receptor type protein-tyrosine kinase that is highly expressed in many hematopoietic cell lines. Hantschel et al. (2007) identified TEC kinase and BTK kinase as major binders of the tyrosine kinase inhibitor dasatinib, which is used for treatment of BCR/ABL-positive CML.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Tec using anti-Tec antibody (A01031).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela cell lysate,
Lane 2: human MCF-7 cell lysate,
Lane 3: human COLO-320 cell lysate,
Lane 4: human HepG2 cell lysate,
Lane 5: rat stomach tissue lysate,
Lane 6: mouse stomach tissue lysate,
Lane 7: mouse heart tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tec antigen affinity purified polyclonal antibody (Catalog # A01031) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tec at approximately 73KD. The expected band size for Tec is at 73KD.
Protein Target Info & Infographic
Gene/Protein Information For TEC (Source: Uniprot.org, NCBI)
Gene Name
TEC
Full Name
Tyrosine-protein kinase Tec
Weight
73.581kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.2; MGC126760; MGC126762; PSCTK4; PSCTK4tyrosine-protein kinase Tec; tec protein tyrosine kinase; Tec TEC PSCTK4 tec protein tyrosine kinase tyrosine-protein kinase Tec
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEC, check out the TEC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tec Antibody Picoband™ (A01031)
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No publications found for A01031
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