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Product Info Summary
| SKU: | A11073 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-TECPR1 Antibody (C-term)
SKU/Catalog Number
A11073
Size
80 µl
Description
Boster Bio Anti-TECPR1 Antibody (C-term) (Catalog # A11073). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TECPR1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11073)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TECPR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1094-1122 amino acids from the C-terminal region of human TECPR1.
Reactive Species
A11073 is reactive to TECPR1 in Human
Calculated molecular weight
129.7 kDa
Background of TECPR1
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11073 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IHC-P: 1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-TECPR1 Antibody (C-term) at 1:500 dilution
Lane 1: HUVEC whole cell lysate
Lane 2: A375 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 130 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of A11073 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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TECPR1 Antibody (C-term) (Cat. #A11073) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-TECPR1 Antibody (C-term) (A11073)
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