Anti-Tectonic-3 TCTN3 Antibody

Product Name

Anti-Tectonic-3 TCTN3 Antibody

View all TCTN3 Antibodies

SKU/Catalog Number

A12051

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Tectonic-3 TCTN3 Antibody (Catalog # A12051). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TCTN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Tectonic-3 TCTN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12051)

Host

Rabbit

Contents

TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3. The immunogen is located within amino acids 540 - 590 of TCTN3.

Reactive Species

A12051 is reactive to TCTN3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

66.2 kDa

Background of TCTN3

TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12051 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TCTN3

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