Product Info Summary
SKU: | A03263-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™
SKU/Catalog Number
A03263-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™ catalog # A03263-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03263-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TEF1/TEAD-1/TEAD1 recombinant protein (Position: D38-D426).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03263-2 is reactive to TEAD1 in Human
Applications
A03263-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
47.946kDa
Background of TEF1
Transcriptional enhancer factor TEF-1 also known as TEA domain family member 1 (TEAD1) and transcription factor 13 (TCF-13) is a protein that in humans is encoded by the TEAD1 gene. This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TEF1/TEAD-1/TEAD1 using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEF1/TEAD-1/TEAD1 antigen affinity purified polyclonal antibody (Catalog # A03263-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEF1/TEAD-1/TEAD1 at approximately 50KD. The expected band size for TEF1/TEAD-1/TEAD1 is at 50KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2).
Overlay histogram showing CACO-2 cells stained with A03263-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TEF1/TEAD-1/TEAD1 Antibody (A03263-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of U87 cells using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2).
Overlay histogram showing U87 cells stained with A03263-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TEF1/TEAD-1/TEAD1 Antibody (A03263-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TEAD1 (Source: Uniprot.org, NCBI)
Gene Name
TEAD1
Full Name
Transcriptional enhancer factor TEF-1
Weight
47.946kDa
Alternative Names
NTEF-1; protein GT-IIC; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1 TEAD1 AA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1 TEA domain transcription factor 1 transcriptional enhancer factor TEF-1|TEA domain family member 1 (SV40 transcriptional enhancer factor)|protein GT-IIC|transcription factor 13|transcriptional enhancer factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEAD1, check out the TEAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™ (A03263-2)
Hello CJ!
No publications found for A03263-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-TEF1/TEAD-1/TEAD1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question