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Product Info Summary
| SKU: | A09540 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-TEM5 ADGRA2 Antibody
SKU/Catalog Number
A09540
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TEM5 ADGRA2 Antibody (Catalog # A09540). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Rat.
Storage & Handling
TEM5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TEM5 ADGRA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09540)
Host
Rabbit
Contents
TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TEM5 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human TEM5. The immunogen is located within amino acids 1270 - 1320 of TEM5.
Reactive Species
A09540 is reactive to GPR124 in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
142.6 kDa
Background of GPR124
Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09540 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TEM5 in human bladder tissue lysate with TEM5 antibody at (A) 2 and (B) 4 μg/mL.
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Immunohistochemistry of TEM5 in human bladder tissue with TEM5 antibody at 5 μg/mL.
Specific Publications For Anti-TEM5 ADGRA2 Antibody (A09540)
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