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Product Info Summary
| SKU: | A00603 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TET1 Antibody
SKU/Catalog Number
A00603
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TET1 Antibody (Catalog # A00603). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TET1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TET1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00603)
Host
Rabbit
Contents
TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TET1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1.
Cross-reactivity
TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.
Reactive Species
A00603 is reactive to TET1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
235.3 kDa
Background of TET1
TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10;11)(q22;q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00603 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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WB Validation of TET1 in Human Cell Lines
Loading: 15 μg of lysate
Antibodies: TET1 A00603, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation of TET1 in Mouse Tissues
Loading: 15 μg of lysate
Antibodies: TET1 A00603 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
WB Validation of TET1 in Rat Tissues
Loading: 15 μg of lysate
Antibodies: TET1 A00603, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of TET1 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with A00603 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
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Immunofluorescence Validation of TET1 in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with A00603 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
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Immunohistochemistry Validation of TET1 in Human Testis Tissue
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (A00603) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (A00603) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of TET1 in Rat Liver Tissue
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (A00603) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-TET1 Antibody (A00603)
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