Anti-TFIIIB90-1 Antibody

Product Name

Anti-TFIIIB90-1 Antibody

View all BRF1 Antibodies

SKU/Catalog Number

A03761-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-TFIIIB90-1 Antibody catalog # A03761-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TFIIIB90-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03761-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

2F1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human TFIIIB90-1. at AA rangle: 200-280

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03761-1 is reactive to BRF1 in Human, Mouse

Applications

A03761-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

73.84kDa

Background of BRF1

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of specific cells using TFIIIB90-1 Polyclonal antibody.

Gene/Protein Information For BRF1 (Source: Uniprot.org, NCBI)

Alternative Names

B-related factor 1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factorIIIB (S. cerevisiae); BRF-1; BRFFLJ42674; general transcription factor IIIB, 90kD subunit; GTF3BTAFIII90; hBRFMGC105048; hTFIIIB90; TAF3B2; TAF3CB - related factor 1; TATA box binding protein (TBP)-associated factor 3C; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3Bsubunit 2; TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; TBP - associated factor, RNA polymerase III BRF1 BRF, BRF-1, CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF BRF1 RNA polymerase III transcription initiation factor subunit transcription factor IIIB 90 kDa subunit|B - related factor 1|BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB|BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit|TATA box binding protein (TBP)-associated factor 3C|TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2|TBP - associated factor, RNA polymerase III, 90kD|epididymis secretory sperm binding protein Li 76p|general transcription factor IIIB, 90kD subunit

For more info on BRF1, check out the BRF1 Infographic

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In this infographic, you will see the following information for BRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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