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Product Info Summary
| SKU: | M01779 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Rabbit, Zebrafish |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody
SKU/Catalog Number
M01779
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (Catalog # M01779). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rabbit, Zebrafish.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01779)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: TG86 + P/T1
Isotype
IgG1, kappa + IgG1, kappa
Immunogen
A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα (TG86 & P/T1)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01779 is reactive to TGFA in Human, Rabbit, Zebrafish
Applications
M01779 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.006kDa
Background of TGF-alpha
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with This monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Anti-TGF alpha Monoclonal Antibody (TG86 + P/T1).
Protein Target Info & Infographic
Gene/Protein Information For TGFA (Source: Uniprot.org, NCBI)
Gene Name
TGFA
Full Name
Protransforming growth factor alpha
Weight
17.006kDa
Alternative Names
protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFA, check out the TGFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (M01779)
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No publications found for M01779
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Customer Reviews
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