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Product Info Summary
| SKU: | A02765-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband®
SKU/Catalog Number
A02765-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband® catalog # A02765-4. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02765-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TGF beta Receptor III/TGFBR3, which shares 92.3% amino acid (aa) sequence identity with mouse and rat TGFBR3.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02765-4 is reactive to TGFBR3 in Human, Rat
Observed Molecular Weight
93 kDa
Calculated molecular weight
93.5 kDa
Background of TGFBR3
Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02765-4 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Rat
Positive Control
WB: human K562 whole cell, human Hela whole cell, rat testis tissue, rat L6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat L6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa.
Specific Publications For Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband® (A02765-4)
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