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- Table of Contents
TGFBR3 · Western blot design guide
TGFBR3 Western Blot Planning Guide
Plan a TGFBR3 Western blot around the catalog-observed 93.5 kDa band, image-backed A02765-5 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
TGFBR3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 93.5 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Testis | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 93.5 kDa |
| ⓘ | Localization | Cell membrane / Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Rat |
Section 1
Real Curated TGFBR3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Placenta |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02765-5 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected TGFBR3 Western Blot Band Size?
Use the product-observed 93.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 93.5 kDa | Matches the authoritative product WB observation. |
| 93.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected TGFBR3 appearancePlan around 93.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 93.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 93.5 kDa from UniProt Q03167; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02765-5. |
| Situation | Likely cause | Next action |
|---|---|---|
| 93.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing TGFBR3 band
A02765-5 · TGFBR3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TGFBR3 Western blot
🧪Use Testis as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Testis (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for TGFBR3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced TGFBR3 Western Blot Tips
Deeper troubleshooting and optimisation questions for TGFBR3, answered from its protein features.
Which band should guide the blot?
Use 93.5 kDa, the observation attached to the authoritative A02765-5 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 93.5 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for TGFBR3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02765-5 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
TGFBR3 Western Blot Reagents
Human/Rat-reactive TGFBR3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband®
Cat # A02765-5
Real WB data
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband®
Cat # A02765-4
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q03167
- Human Protein Atlas — TGFBR3
- A02765-5 product record
- PMC6000709 — [Defective expression of TGFBR3 gene and its molecular mechanisms in non-small cell lung cancer cell lines] (Zhongguo fei ai za zhi = Chinese journal of lung cancer, 2010)
- PMC9524034 — LncRNA H19 alleviates sepsis-induced acute lung injury by regulating the miR-107/TGFBR3 axis (BMC pulmonary medicine, 2022)
- PMC9313949 — Long Noncoding RNA Zinc Finger Antisense 1 Affects Glucocorticoid-Induced Osteonecrosis of the Femoral Head by Performing as a ceRNA for MicroRNA-124-3p and Accelerating Transforming Growth Factor Type III Receptor (Computational and mathematical methods in medicine, 2022)