Click image to see more details
Product Info Summary
| SKU: | M02229-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02229-1
BM5139 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal Antibody catalog # M02229-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02229-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABEG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TGM2
Reactive Species
M02229-1 is reactive to TGM2 in Human, Mouse
Observed Molecular Weight
77 kDa
Calculated molecular weight
77.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02229-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: HUVEC cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TGM2 expression in HUVEC cell lysate.
Specific Publications For Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal Antibody (M02229-1)
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Customer Q&As
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7 Customer Q&As for Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal Antibody
Question
Will M02229-1 anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, M02229-1 anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
Would anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 work for IP with liver?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
According to the expression profile of liver, TGM2 is highly expressed in liver. So, it is likely that anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 will work for IP with liver.
Boster Scientific Support
Answered: 2019-10-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
Does anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
Our lab technicians have not tested anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-06
Question
My question regards to test anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 on human liver for research purposes, then I may be interested in using anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
The products we sell, including anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-10
Question
I see that the anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-10
Question
I have attached the WB image, lot number and protocol we used for liver using anti-TGM2/Transglutaminase 2 Rabbit Monoclonal antibody M02229-1. Please let me know if you require anything else.
A. Jha
Verified customer
Asked: 2017-03-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-20
